Andrew L Lee, Ph.D.

Division Director of Graduate Admissions Professor

Research

The role of protein dynamics in function, especially allostery, conformational changes, enzyme catalysis, and drug/ligand binding; mechanisms of PDZ domain function and regulation; NMR spectroscopy; protein biophysics.

Andrew Lee received his BA in chemistry at Pomona College in 1989. For his PhD work at UC Berkeley (supervised by David E. Wemmer), he studied NMR spectroscopy as a method for structure determination of RNA-binding proteins. For his postdoctoral work at the University of Pennsylvania (with A. Joshua Wand), he studied the use of NMR relaxation methods for probing the role of motional dynamics in protein stability and molecular recognition.

Research in the Lee laboratory is centered on enhancing understanding of protein function from the perspective of physical structural biology. In past decades, proteins were essentially viewed as static structures. Today, they are widely appreciated to be dynamic ensembles of interconverting structures. Such behavior can be clearly seen in proteins that undergo dramatic shape changes in different functional states. However, the effects of dynamics can also be important when the motions are “less coordinated” structurally and occur within the original protein shape. The ensemble nature of proteins has far-reaching implications for understanding basic natural protein functions such as ligand binding, enzyme catalysis, and allostery. By extension, an understanding of protein dynamics should lead to improvements in protein engineering and rational drug design.

Lee’s group is investigating the role of structural dynamics in protein function for a variety of proteins important in metabolism and signal transduction. This includes enzymes such as dihydrofolate reductase (DHFR), which is essential in all organisms for nucleotide biosynthesis. It is also the target for drugs used to treat human cancers and antibiotics and is therefore an excellent system for studying protein-drug interactions. Another class of proteins in which we are interested is the PDZ (PSD95 – Discs Large – ZO-1) domain. These are found throughout nature in a variety of contexts and are widespread as signal transduction modules in higher eukaryotes. PDZ domains specialize in binding C-terminal sequences of signaling enzymes, transmembrane receptors, proteases, etc. They are also recognized as models for intramolecular communication. Our NMR studies on PDZ domains implicate dynamics as a viable mechanism for long-range communication and allostery. Other ongoing projects in the lab are directed at the role of protein dynamics in allostery and their distinguishing features from non-allosteric proteins.

How does Lee characterize protein dynamics? Thee preferred method is heteronuclear NMR spectroscopy, which is uniquely suited to study both structure and dynamics in proteins and other biological macromolecules. A major advantage of NMR is that spectroscopic probes are distributed uniformly throughout the biomolecule, such as NH or CH atom pairs, providing large amounts of molecular information. To gain information on protein dynamics, NMR spin relaxation is highly sensitive to molecular motion over a range of timescales. Lee’s group looks at the relaxation properties of 15N, 13C, and 2H spins located throughout the protein scaffold, and interpret these in terms of amplitudes and timescales of individual bond vectors. Slower motions on the microsecond-millisecond timescale can be detected to yield site-specific kinetic, thermodynamic, and structural information on the switching between discrete conformational states. In many cases, these NMR-relaxation dynamics are used to complement other structural data from X-ray crystallography, or thermodynamic and kinetic biophysical measurements using methods such as fluorescence spectroscopy, calorimetry, amide hydrogen exchange, and molecular dynamics simulations.

  • Postdoctoral, University of Pennsylvania, 2001
  • PhD, Chemistry University of California, Berkeley, 1996
  • BA, Chemistry Pomona College, 1989

Lee’s School News

  • Andrew Lee

    Andrew Lee Promoted to Full Professor

    The UNC Eshelman School of Pharmacy has promoted Andrew Lee, PhD, to full professor. A faculty member in the Division of Chemical Biology and Medicinal Chemistry, Lee joined the School in 2001 as an assistant professor and was promoted to … Continued

  • Lee Receives NIH Grant for Collaboration with Singleton

    Andrew Lee, PhD, an associate professor at the UNC School of Pharmacy, has received a grant from the National Institutes of Health for a collaboration with fellow School faculty Scott Singleton, PhD, to study at how protein motions affect function … Continued