Publications

Independent Research

Published (reverse chronological order)

  1. Lee, A.M., and Singleton, S.F., “A Molecular Target for Suppression of the evolution of Antibiotic Resistance: Inhibition of the Escherichia coli RecA Protein by N6-(1-Naphthyl)-ADP,” Journal of Medicinal Chemistry, 48: 5408-5411 (2005).
  2. Lee, A.M., and Singleton, S.F., “Inhibition of the Escherichia coli RecA protein: zinc(II), copper(II) and mercury(II) trap RecA as inactive aggregates,” Journal of Inorganic Biochemistry, 98: 1981-1986 (2004).
  3. Roca, A.I., and Singleton, S.F., “Direct Evaluation of a Mechanism of Activation of the RecA Nucleoprotein Filament,” Journal of the American Chemical Society, 125: 15366-15375 (2003).
  4. Singleton, S.F., Simonette, R.A., Sharma, N.C., and Roca, A.I., “Intein-Mediated Affinity-Fusion Purification of the Escherichia coli RecA Protein,” Protein Expression & Purification, 26: 476-488 (2002).
  5. Xiao, J., and Singleton, S.F., “Elucidating a Key Intermediate in Homologous DNA Strand Exchange:  Structural Characterization of the RecA·Triple-Stranded-DNA Complex Using Fluorescence Resonance Energy Transfer,” Journal of Molecular Biology, 320: 529-558 (2002).
  6. Singleton, S.F., and Xiao, J., “The Stretched DNA Geometry of Recombination and Repair Nucleoprotein Filaments,” Biopolymers (Nucleic Acid Science), 61: 145-158 (2002).
  7. Peng, Z.-H., Sharma, V., Singleton, S.F., and Gershon, P.D., “Synthesis and Application of a Chain-Terminating Dinucleotide mRNA Cap Analog,” Organic Letters, 4: 161-164 (2002).
  8. Singleton, S.F., Shan, F., Kanan, M.W., McIntosh, C.M., Stearman, C.J., Helm, J.S., and Webb, K.J., “Facile Synthesis of a Fluorescent Deoxycytidine Analogue Suitable for Probing the RecA Nucleoprotein Filament,” Organic Letters, 3: 3919-3922 (2001).
  9. Berger, M.D., Lee, A.M., Simonette, R.A., Jackson, B.E., Roca, A.I., and Singleton, S.F., “Design and Evaluation of a Tryptophanless RecA Protein with wild type Activity,” Biochemical and Biophysical Research Communications, 286: 1195-1203 (2001).


Submitted for publication.

  1. Lee, A.M. and Singleton, S.F., “Intersubunit Electrostatic Complementarity in the RecA Nucleoprotein Filament Regulates Nucleotide Substrate Specificity and Conformational Activation,” to Biochemistry.
  2. Wigle, T.J., Lee, A.M., and Singleton, S.F., “Conformationally Selective Binding of Nucleotide Analogs to Escherichia coli RecA:  A Ligand-Based Analysis of the RecA ATP-Binding Site,” to Biochemistry.
  3. Xiao, J., Lee, A.M., and Singleton, S.F., “Construction and Evaluation of a Kinetic Scheme for RecA-mediated DNA Strand Exchange,” to Biopolymers.

 

 In preparation.

  1. Xiao, J., Lee, A.M., and Singleton, S.F., “Nucleic Acid Dynamics During Homologous Genetic Recombination: Entropy Trapping in the RecA Mechanistic Strategy,” for ChemBioChem.
  2. Lee, A.M., Xiao, J., and Singleton, S.F., “Direct Evaluation of a Kinetic Model for RecA-mediated DNA Strand Exchange:  Insight into the Origins of Sequence Selectivity in Homologous Genetic Recombination,” for Nature Structural and Molecular Biology.
  3. Sharma, N.C., Simonette, R.A., and Singleton, S.F., “A Semi-synthetic Genetic Circuit for the Selection of Rare, Site-specific Proteases from Gigascale Libraries,” for Nature Methods.
  4. Sharma, N.C., and Singleton, S.F., “Rapid Activity Screens for Human Viral Proteases in Escherichia coli,” for Protein Engineering.
  5. Wigle, T.J. and Singleton, S.F., “Functionally Selective RecA Ligands for Modulating the Bacterial Genomic Repair and Recombination Machinery,” for Journal of the American Chemical Society.
  6. Cline, D.J., and Singleton, S.F., “Inhibition of the Escherichia coli RecA Protein by Designed, Synthetic Mimetics of its N-terminal Helix,” for Organic and Biomolecular Chemistry.
  7. Wigle, T.J., Zeng, B.-B., and Singleton, “Bismuth-dithiol Inhibition of the Escherichia coli RecA Protein,” for Journal of Inorganic Biochemistry.
  8. Hadimani, M.B., Lee, A.M., and Singleton, S.F., “Cyclo-Sal Pronucleotides of AZT: Antibacterial Agents that Bypass Drug Resistance,” for Bioorganic and Medicinal Chemistry.
  9. Berger, M.D., and Singleton, S.F., “Steady-state Fluorescence of the Escherichia coli RecA Protein:  Analysis Using Single-Tryptophan Probes,” for Biophysical Journal.
  10. Berger, M.D., Lee, A.M., Simonette, R.A., Patel, S., Dalton, K.E., and Singleton, S.F., “The Role of the Conserved Tryptophan 290 in the RecA C-terminal Domain,” for Nucleic Acids Research.

Undergraduate, Graduate and Postdoctoral Research

Published (reverse chronological order).

  1. Cannon, W.R., Singleton, S.F., and Benkovic, S.J., “A Perspective on Biological Catalysis,” Nature Structural Biology, 3: 821-833 (1996).
  2. Wagner, C.R., Huang, Z., Singleton, S.F., and Benkovic, S.J., “The Molecular Basis for Non-Additive Mutational Effects in Escherichia coli Dihydrofolate Reductase,” Biochemistry, 34: 15671-15680 (1995).
  3. Plum, G.E., Pilch, D.S., Singleton, S.F., and Breslauer, K.J., “Nucleic Acid Hybridization: Triplex Stability and Energetics,” Annual Reviews of Biophysics and Biomolecular Structure, 24: 319–350 (1995).
  4. Singleton, S.F., and Dervan, P.B., “Temperature Dependence of the Energetics of Oligonucleotide-Directed Triple Helix Formation at a Single DNA Site,” Journal of the American Chemical Society, 116: 10376–10382 (1994).
  5. Singleton, S.F., and Dervan, P.B., “Equilibrium Association Constants for Oligonucleotide-Directed Triple Helix Formation at Single DNA Sites:   Linkage to Cation Valence and Concentration,” Biochemistry, 32: 13171–13179 (1993).
  6. Singleton, S.F., and Dervan, P.B., “Influence of pH on the Equilibrium Association Constants for Oligodeoxyribonucleotide-Directed Triple Helix Formation at Single DNA Sites,” Biochemistry, 31: 10995–11003 (1992).
  7. Singleton, S.F., and Dervan, P.B., “Thermodynamics of Oligonucleotide-Directed Triple Helix Formation:  An Analysis Using Quantitative Affinity Cleavage Titration,” Journal of the American Chemical Society, 114: 6957–6965 (1992).
  8. Plum, G.E., Park, Y.-W., Singleton, S.F., and Dervan, P.B., “Thermodynamic Characterization of the Stability and the Melting Behavior of a DNA Triplex:  A Spectroscopic and Calorimetric Study,” Proceedings of the National Academy of Sciences U.S.A., 87: 9436–9440 (1990).
  9. Plummer, B.F., and Singleton, S.F., “An Analysis of the Electronic Transitions of Aceanthrylene,” Journal of Physical Chemistry, 94: 7363–7366 (1990).
  10. Plummer, B.F., and Singleton, S.F., “Triplets, Biradicals, Radical Ions, and the Heavy-Atom Solvent Effect in the Photodimerization of Aceanthrylene,” Journal of Physical Chemistry , 93: 5515–5520 (1989).
  11. Plummer, B.F., and Singleton, S.F., “The Photodimerization of Aceanthrylene,” Tetrahedron Letters , 28: 4801–4804 (1987).
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